Composite

Part:BBa_K3838222:Design

Designed by: Siyang Yu   Group: iGEM21_SZU-China   (2021-08-30)


J-BSH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 969
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The bacteria nissle 1917 and DH5α will express the gene itself.So the provement of this part should be considered.


Source

the BSH gene is from Lactobacillus plantarum strain MBUL90.

References